Journal: Cell Biology and Toxicology

Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia

doi: 10.1007/s10565-026-10170-7

Figure Lengend Snippet: Alteration of decidual NK and macrophage expression levels of SPP1 and CCL pathway associated genes in PE patients. ( A ) Bar plot ranking significant signaling pathways based on differences in overall information flow within inferred cell-cell communication networks between PE and control groups. ( B ) Volcano plot displaying DEGs in dMacro from PE patients compared to Controls. ( C ) UMAP visualization and bar plot of decidua macrophage(dMacro), identifying three major subclusters. ( D ) Violin plots comparing signature scores of dMacro between PE and Control groups, specifically for phagocytosis (GO:0006909). **** p < 0.0001. ( E ) Confocal laser scanning microscopy (CLSM) images showing the secretion of PTN and OGN in peripheral natural killer (pNK) cells, decidual natural killer (dNK) cells, and induced NK (iNK) cells. Scale bars, 10 µm. ( F ) mRNA expression levels of CCL3, CCL4, CCR1, SPP1, and CD44 in decidual tissue from PE (n = 6) and Control (n = 6) samples. Data are presented as mean ± SD, unpaired t test was used for comparisons among two groups. Statistical significance: ** p < 0.01, **** p < 0.0001. ( G ) Circular diagram illustrating cell-cell interactions mediated by the CCL and SPP1 pathways in PE and Control groups. ( H ) Flow cytometry analysis of the percentage of PRF1, GZMA, GZMB, and GNLY expression in iNK cells cultured with or without SPP1(n=5). Data are presented as mean ± SD, unpaired t test was used for comparisons among two groups. Statistical significance: * p < 0.05

Article Snippet: To investigate SPP1's effects on cytotoxic function, differentiated decidual-like NK cells were replated in 24-well plates at 1–1.5 × 106 cells/mL and divided into two groups: 1 Experimental group: Treated with 2 μg/mL recombinant human SPP1 (MedChemExpress; Cat# HY- P70499 ); 2 Control group: Received equivalent volume of sterile double-distilled water.

Techniques: Expressing, Protein-Protein interactions, Control, Confocal Laser Scanning Microscopy, Flow Cytometry, Cell Culture

Journal: Scientific Reports

Article Title: Propolis nanoemulsion and mesenchymal stem cell conditioned medium promote osteoblastogenesis against lipopolysaccharide-induced osteolysis in hyperglycemic rats

doi: 10.1038/s41598-026-46778-2

Figure Lengend Snippet: Serum concentrations of (a) ALP, (b) Coll1a1, and (c) osteopontin across treatment groups. Significant differences between groups are indicated above the bars. Abbreviations: ALP, alkaline phosphatase; Coll1a1, collagen type I alpha 1; LPS, lipopolysaccharide; NEP, nanoemulsion propolis; HUCMSCs-CM, human umbilical cord mesenchymal stem cells conditioned medium. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: This was performed following the instructions of the rat Coll1a1, ALP, and osteopontin ELISA kit (BT LAB, Shanghai Korain Biotech Co., Ltd., Shanghai, China).

Techniques: